RNA
Part:BBa_K4642022:Design
Designed by: Ryan Yapa Group: iGEM23_City-of-London-UK (2023-09-28)
Gen4 373-21-24 Double AND-Gate Toehold Switch Anti-miRNA 24-21
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We had to ensure the anti-mIRNA alone does not bind to the toehold switch.
Source
Custom designed in-silico using NUPACK API.